Pemanfaatan Buah Bit (Beta vulgaris L.) sebagai Pengganti Eosin pada Pewarnaan Papanicolaou

Lisa Ramadanti; Indah Sari; Aristoteles; Nyiayu Fauziah Kurniawati

doi:10.52523/jhast.v4i2.105

Authors

Lisa Ramadanti Universitas Muhammadiyah Ahmad Dahlan Palembang
Indah Sari IKesT Muhammadiyah Palembang
Aristoteles Universitas Muhammadiyah Ahmad Dahlan Palembang
Nyiayu Fauziah Kurniawati Universitas Sriwijaya Dahlan Palembang

DOI:

https://doi.org/10.52523/jhast.v4i2.105

Keywords:

eosin, beetroot, pleura, papanicolaou

Abstract

Background: The pleura is a serous membrane that folds on the surface of the lungs to form a two-layer membranous structure. The gold standard in establishing the diagnosis of pleural effusion is cytological examination. An important stage in cytological examination is the staining process, where the staining technique uses eosin. Eosin as a dye component is carcinogenic and poses a risk to health and the environment if used in the long term. Beetroot containing red pigment, which is a betacyanin compound, was developed as a natural alternative to eosin. Research Objective: This study aims to determine the use of Beetroot (Beta vulgaris L.) as a substitute for eosin in Papanicolaou staining. Research Method: This is an experimental study with an Intact Group Comparison design consisting of two treatment groups, namely staining using eosin and beetroot. Data analysis was carried out using descriptive statistics. This study was conducted at the Barokah Palembang anatomical pathology laboratory on March 16-17, 2025. The population taken was pleural effusion fluid preparations located at the Barokah Palembang anatomical pathology laboratory, the samples used in this study amounted to 32 samples calculated using the Federer formula. Results: : The results of staining using eosin showed that the quality of the preparation was 100% very good, while for staining using beetroot, the quality of the preparation was not good at 18.7%, less good at 50.0%, and good at 31.2%. Conclusion: Alternative staining beetroot can be made into a coloring alternative to the eosin substitute in Papanicolaou coloring.

References

Hayuningrum D fitri. Diagnosis Efusi Pleura. J Penelit Perawat Prof. 2020;2(4):529–36.

Wijayanti AN, Mahtuti EY, Basyarudin M. Perbandingan Proses Clearing Antara Xylol dan Minyak Gandapura Terhadap Kualitas Preparat Awetan Kutu Tikus ( Xenopsylla Cheopis ) Sekolah Tinggi Ilmu Kesehatan Maharani Malang , Indonesia Universitas Islam Negri Malang , Indonesia. J Ilmu Kesehat. 2024;1(4):44–53.

Mamay, Ernawati, Nurisani A. Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining. Indones J Med Lab Sci Technol. 2023;5(2):100–11.

Amelia agdes, Andriani Y, Andriani L. GAMBARAN HISTOPATOLOGI OTAK MENCIT (Mus musculus L) SETELAH PEMBERIAN FRAKSI DAUN SEMBUNG RAMBAT (Mikania micrantha Kunth) SEBAGAI AKTIVITAS NEUROPROTEKTAN. J Farmamedika (Pharmamedica Journal). 2020;5(1):30–7.

Permatasari R, Fuji Verdian Putra, Silvia Maharani. Potensi Buah Delima Merah (Punica Granatum L.) Sebagai Pewarnaan Alternatif Pengganti Eosin pada Pewarnaan Papanicolaou. SEHATMAS J Ilm Kesehat Masy. 2023;2(1):288–95.

Jannah BP dan L miftahul. Metodologi Penelitian Kuantitatif. Vol. 3, PT Rajagrafindo Persada. 2016. 57 p.

Sukeksi A, Teguh Isworo J, Alvionita D, Putri T. Pengaruh Waktu Pengecatan Menggunakan Ekstrak Kayu Secang (Caesalpinia sappan) Terhadap Warna Sel Eritrosit Pada Sediaan Apusan Darah Tepi (SADT). Pros Semin Nas UNIMUS. 2022;5:1482–9.

Apriani, Andrianus, Marisca S, Diana P. Ez Prep Concentrate (Ez Prep) Sebagai Alternatif Reagen Deparafinasi Pada Pewarnaan Hematoksilin Eosin. G-Tech J Teknol Terap. 2023;7(1):96–102.

Haliza Nur et al. PEMANFAATAN EKSTRAK BUNGA ASOKA SEBAGAI Utilization of Ashoka Flower Extract as an Alternative to Eosin in Hematoxylin. J Ris Kesehat POLTEKKES DEPKES BANDUNG. 2025;17(1):100–6.

Maimunah S, Amila A, Kenedy Marpaung J, Irennius Girsang V, Syapitri H. KARAKTERISASI DAN SKRINING FITOKIMIA DARI TEPUNG BUAH BIT (Beta vulgaris L.). Forte J. 2021;1(2):139–45.

Adhe Bianggo NauE D, Syailendra A, Syafitri I, Wulandari S, Julianti W. BUAH BIT (Beta vulgaris L.) SEBAGAI ALTERNATIF SAFRANIN PADA PEWARNAAN GRAM. Husada Mahakam J Kesehat. 2022;12(1):19–24.

Niswah AA, Bintari YR, Risandiansyah R. Piper betle L. sebagai pewarna bakteri: Uji akurasi dan presisi warna pada Staphylococcus aureus dan Escherichia coli. J Kedokt Komunitas. 2022;10(2):1–10.

Kartini S, Angelia E. Utilization of Juice Beta vulgaris. L as an Alternative Reagent for Examination of Worm Eggs Ascaris lumbricoides. JPK J Prot Kesehat. 2021;10(1):20–5.

Suraini, Sophia A. Optimasi Air Perasan Ubi Jalar Ungu Ipome batatas L. Pada Pemeriksaan Telur Cacing. Bioma J Biol Makasar [Internet]. 2022;7(2):8–13. Available from: https://journal.unhas.ac.id/index.php/bioma

Rahmawati F, Purwaning Sari K, Huda N, Rousdy DW. Ekstrak Biji Kesumba Keling (Bixa orellana L.) sebagai Pewarna Alami Sediaan Jaringan Ikan Nila (Oreochromis niloticus). J Bios Logos. 2023;13(3):233–42.