POTENSI KACANG KEDELAI (Glycine max (L.) Merrill) SEBAGAI MEDIA KULTUR ALTERNATIF TERHADAP PERTUMBUHAN Candida sp

Erwin edyansyah; Dian Adhe Bianggo Naue; Asrori

doi:10.52523/jhast.v4i2.116

Authors

Erwin edyansyah Poltekkes Kemenkes Palembang
Dian Adhe Bianggo Naue Poltekkes Kemenkes Palembang
Asrori Poltekkes Kemenkes Palembang

DOI:

https://doi.org/10.52523/jhast.v4i2.116

Keywords:

Candida sp., Glycine max, alternative culture media. soybean

Abstract

Abstract

Background: Sabouraud Dextrose Agar (SDA) is a standard medium widely used for the isolation and growth of fungi. However, its relatively high cost and reliance on commercial media have encouraged the development of alternative media based on natural ingredients. Soybeans (Glycine max (L.) Merrill) have a high protein and carbohydrate content, making them a potential source of nutrients in fungal culture media. Objective: This study aims to determine the potential of soybeans (Glycine max (L.) Merrill) as an alternative culture medium for the growth of Candida sp. Method: This study used a quasi-experimental method. The culture medium was made from soybeans with varying concentrations of 10%, 20%, 30%, 40%, 50%, and 60%. Each medium was inoculated with Candida sp. isolates and incubated at 28–30°C. Results: The diameter of the colonies formed increased gradually, from 1.4 mm at a concentration of 10% to 2.6 mm at a concentration of 60%. Increasing the concentration of soybean extract was followed by an increase in colony diameter. Conclusion: Soybean (Glycine max (L.) Merrill)-based media can support the growth of Candida sp. Thus, soybean media have the potential to be a more economical alternative culture medium, although further development and standardization are required to match the performance of Sabouraud Dextrose Agar.

References

V. M. Cooke, R. J. Miles, R. G. Price, G. Midgley, W. Khamri, and A. C. Richardson, “New chromogenic agar medium for the identification of Candida spp.,” Appl. Environ. Microbiol., vol. 68, no. 7, pp. 3622–3627, 2002, doi: 10.1128/AEM.68.7.3622-3627.2002.

M. C. Fisher and D. W. Denning, “The WHO fungal priority pathogens list as a game-changer,” Apr. 01, 2023, Nature Research. doi: 10.1038/s41579-023-00861-x.

F. Wang, J. Zhang, Q. Zhang, Z. Song, and C. Xin, “Antifungal activities of Equol against Candida albicans in vitro and in vivo,” Virulence, vol. 15, no. 1, 2024, doi: 10.1080/21505594.2024.2404256.

S. G. A. Al-Saffy and D. A. Abdulshaheed, “Isolation and identification of Aspergillus fumigatus from feline respiratory infection in Baghdad province,” Int. J. Health Sci. (Qassim)., pp. 13910–13915, Jun. 2022, doi: 10.53730/ijhs.v6ns2.8648.

D. Derkacz, P. Bernat, and A. Krasowska, “K143R Amino Acid Substitution in 14-α-demethylase (Erg11p) Changes Plasma Membrane and Cell Wall Structure of Candida albicans,” Int. J. Mol. Sci., vol. 23, no. 3, Feb. 2022, doi: 10.3390/ijms23031631.

M. Katsipoulaki, M. H. T. Stappers, D. Malavia-Jones, S. Brunke, B. Hube, and N. A. R. Gow, “ Candida albicans and Candida glabrata : global priority pathogens ,” Microbiology and Molecular Biology Reviews, vol. 88, no. 2, Jun. 2024, doi: 10.1128/mmbr.00021-23.

T. Scognamiglio, R. Zinchuk, P. Gumpeni, and D. H. Larone, “Comparison of inhibitory mold agar to sabouraud dextrose agar as a primary medium for isolation of fungi,” J. Clin. Microbiol., vol. 48, no. 5, pp. 1924–1925, May 2010, doi: 10.1128/JCM.01814-09.

Rafika Rafika et al., “Rice Bran Dextrose Agar as a Cost-Effective Alternative to Standard Fungal Culture Media,” Tropical Journal of Natural Product Research, vol. 9, no. 12, Jan. 2026, doi: 10.26538/tjnpr/v9i12.39.

I. S. Kim, “Current perspectives on the beneficial effects of soybean isoflavones and their metabolites on plants,” May 01, 2022, The Korean Society of Food Science and Technology. doi: 10.1007/s10068-022-01070-7.

Rahayu, P. S., Solikah, M. P., & Shafriani, N. R. Uji efektivitas konsentrasi kacang kedelai sebagai media alternatif terhadap pertumbuhan jamur Candida albicans. Anakes: Jurnal Ilmiah Analis Kesehatan. Vol. 11 No. 1; Maret 2025 p-ISSN: 2088-5687 e:ISSN: 2745-6099.

F. Fitrotul Azlina, E. Puspitasari, Q. A’yunin Lathifah, and I. Putri, “Differences in the growth of Candida albicans fungi on alternative media of soybean (Glycine max (L.) Merrill) and Sabouraud Dextrose Agar (SDA),” Journal of Science Education Literacy, vol. 1, no. 1, 2026, doi: 10.30872/jsel.v1i1.6520.

J. Kesehatan Saelmakers PERDANA, M. Ulva, U. Muhammadiyah Ahmad Dahlan Palembang, and S. Selatan Indonesia, “Ulva dkk Perbedaan Pertumbuhan Jamur Cryptococcus neoformans Pada Media Sabouraud Dextrose Agar (SDA) dan Media Alternatif Buah Nipah (Nypa fruticans) Differences in the Growth of Cryptococcus neoformans Fungus on Sabouraud Dextrose Agar (SDA) Media and Alternative Nipah Fruit Media (Nypa fruticans),” 462 | JKSP, vol. 8, no. 2, 2025, doi: 10.32524/jksp.v8i2.1506.

A. Nuryati, S. Analis, K. Poltekkes, and K. Yogyakarta, “Media Agar Tepung Kacang Hijau, Kacang Merah, Kacang Tunggak, Kacang Kedelai Sebagai Media Kultur Jamur Aspergillus flavus.”

U. Urip, Y. Jiwintarum, and N. L. P. G. Gandi, “Studi Jamur Aspergillus fumigatus di Pasar Cakranegara Kota Mataram Penyebab Penyakit Aspergillosis Menggunakan Media Pertumbuhan Potato Dextrose Agar,” Bioscientist : Jurnal Ilmiah Biologi, vol. 9, no. 2, p. 631, Dec. 2021, doi: 10.33394/bioscientist.v9i2.4560.

M. C. Fisher and D. W. Denning, “The WHO fungal priority pathogens list as a game-changer,” Apr. 01, 2023, Nature Research. doi: 10.1038/s41579-023-00861-x.

W. Zhang, H. Zhang, M. Zhan, R. Jing, X. Wang, and Z. Zhang, “Epidemiological characteristics of invasive Aspergillus isolates: Morphology, drug susceptibility, and mutations in azole drug targets,” J. Infect. Public Health, vol. 18, no. 1, Jan. 2025, doi: 10.1016/j.jiph.2024.102612.

Michael T. Madigan, Kelly S. Bender, and W Matthew Sattley, Brock Biology of Microorganisms, 16th edition. Pearson, 2020.

Y. Suryani and O. Taupiqurrohman, Mikrobiologi Book, vol. 75(17). Pharmacognosy Magazine, 2021.

Y. Kusumo, A. A. Atmanto, L. Amin Asri, N. A. Kadir, D. Spesialis, and P. Klinik, “MEDIA PERTUMBUHAN KUMAN.” [Online]. Available: http://jurnalmedikahutama.com

Y. Kristianingsih et al., “Analisis Candida albicans Pada Air Dalam Bak Toilet SMA Negeri 48 Jakarta Timur,” Maret 2023 p-Open Journal System (OJS): journal.thamrin.ac.id, vol. 9, no. 1, [Online]. Available: http://journal.thamrin.ac.id/index.php/anakes/issue/view/83

J. Lei et al., “Antifungal activity of vitamin D3 against Candida albicans in vitro and in vivo,” Microbiol. Res., vol. 265, Dec. 2022, doi: 10.1016/j.micres.2022.127200.