The Effectiveness of Neutral Buffered Formalin in Preserving Urine Erithrocytes with Delayed Testing

Mardyana Nizar; Trisna Citra

doi:10.52523/jhast.v4i2.120

Authors

Mardyana Nizar Poltekkes Kemenkes Palembang
Trisna Citra Poltekkes Kemenkes Banten

DOI:

https://doi.org/10.52523/jhast.v4i2.120

Keywords:

netral buffer formalin, eritrosit urin, fiksatif

Abstract

Urine erythrocyte examination is one of the important parameters in urinalysis to detect hematuria and abnormalities of the urinary system. Delays in examination often become a challenge in laboratories, as erythrocytes are prone to lysis or morphological changes that may affect result accuracy. This study aimed to determine the effectiveness of Neutral Buffered Formalin (NBF) as a fixative in maintaining the count and morphology of urine erythrocytes at different examination delay times: 0 hours as control, and delays of 3 hours and 6 hours.

Urine samples were collected using purposive sampling, and erythrocyte sediment examination was performed using a light microscope. Data analysis employed the Dependent Sample T-Test to compare results across each delay interval.

The findings showed that the use of NBF was able to maintain the stability of urine erythrocytes up to a 6-hour delay, with differences in count and morphology that were not statistically significant (p > 0.05). These results indicate that NBF is effective as a fixative to preserve the quality of urine erythrocyte examination despite delays in analysis.

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