The Effectiveness of Neutral Buffered Formalin in Preserving Urine Erithrocytes with Delayed Testing

Mardyana Nizar; Trisna Citra

doi:10.52523/jhast.v4i2.120

Penulis

Mardyana Nizar Poltekkes Kemenkes Palembang
Trisna Citra Poltekkes Kemenkes Banten

DOI:

https://doi.org/10.52523/jhast.v4i2.120

Kata Kunci:

netral buffer formalin, eritrosit urin, fiksatif

Abstrak

Pemeriksaan eritrosit urin merupakan salah satu parameter penting dalam urinalisis untuk mendeteksi adanya hematuria maupun kelainan sistem kemih. Keterlambatan pemeriksaan sering menjadi kendala di laboratorium, karena eritrosit mudah mengalami lisis atau perubahan morfologi sehingga dapat memengaruhi akurasi hasil. Penelitian ini bertujuan untuk mengetahui efektivitas Netral Buffer Formalin (NBF) sebagai fiksatif dalam mempertahankan jumlah dan morfologi eritrosit urin pada variasi penundaan waktu pemeriksaan 0 jam sebagai kontrol, dan variasi waktu penundaan 3 jam, dan 6 jam. Sampel urin dikumpulkan dengan metode purposive sampling, kemudian dilakukan pemeriksaan sedimen eritrosit menggunakan mikroskop cahaya. Analisis data menggunakan uji Dependent Sample T-Test untuk membandingkan hasil pemeriksaan pada tiap interval penundaan.Hasil penelitian menunjukkan bahwa penggunaan NBF mampu mempertahankan stabilitas eritrosit urin hingga penundaan 6 jam, dengan perbedaan jumlah dan morfologi yang tidak signifikan secara statistik (p > 0,05). Temuan ini mengindikasikan bahwa NBF efektif digunakan sebagai fiksatif untuk menjaga kualitas pemeriksaan eritrosit urin meskipun terjadi penundaan waktu analisis

Referensi

Rusmini, R., & Ridhoni, M. A. (2026). Optimization of urine storage with the addition of 40% formalin at 2–8°C: Stability of sediment elements. Tropical Health and Medical Research, 8(1). https://doi.org/10.35916/thmr.v8i1.144

Fujieda, K., Furuhashi, K., Watanabe, K., et al. (2025). Novel fixative solutions with superior preservation compared to 10% NBF. Nagoya Journal of Medical Science, 88(2),182–193. https://doi.org/10.18999/nagjms.88.2.182 (doi.org in Bing)

Taha, S., Ismaeel, A., Aljishi, M., et al. (2025). Silver nanoparticles as a novel tissue preservative: A comparative study with 10% NBF. International Journal of Molecular Sciences, 26(11), 5335. https://doi.org/10.3390/ijms26115335 (doi.org in Bing)

Nonkes, L., Yaz Aydın, G., Van’t Hof, M., & Demir, A. Y. (2023). Impact of different preservation methods on urinary red blood cell counts. Clinical Chemistry and Laboratory Medicine. https://doi.org/10.1515/cclm-2023-0892 (doi.org in Bing)

Sari, D. A. I. (2023). Differences in urine sediment results preserving formalin and toluene. Jurnal Laboratorium Khatulistiwa, 6(2). https://doi.org/10.30602/jlk.v6i2.566

Freund, P., Goerlich, N., Skopnik, C. M., et al. (2023). Formaldehyde releaser and buffer enable delayed urine cell analysis. Cytometry Part B: Clinical Cytometry, 104(4), 417–425. https://doi.org/10.1002/cyto.b.22117 (doi.org in Bing)

Ndengue, C. P., Atangana, P. J. A., Ateba, G. R., et al. (2026). Pre-analytical variables affecting biomarker expression: Neutral buffered formalin vs non-buffered. PLOS One. https://doi.org/10.1371/journal.pone.0343185

Rideg, O., Dergez, T., Tóth, A., et al. (2025). Validation of ancillary procedures on formalin-fixed cytologic samples. American Journal of Clinical Pathology, 164(6), 924–932. https://doi.org/10.1093/ajcp/aqaf117

Rahmi, M., Hutasoit, G. A., Fitriani, J., & Basry, A. (2026). Effect of 10% NBF fixation on uterine mesenchymal cell morphology. Medika Tadulako, 11(1), 1–7. https://doi.org/10.22487/f5710y30

Apriyani, R. K., & Melani, M. S. (2023). Leukocyte counts in urine samples with different storage times. Jurnal Kesehatan Tambusai, 4(3), 3238–3245. https://doi.org/10.31004/jkt.v4i3.3238 (doi.org in Bing)

Johnson, R., Patel, A., & Chen, Y. (2021). Simple urine storage protocol for extracellular vesicle proteomics. Proteomics Clinical Applications, 15(5), e210002. https://doi.org/10.1002/prca.202100002 (doi.org in Bing)

Salazar-García, S., Lares-Villaseñor, E., Bárcenas-Morales, A., et al. (2023). Impact of chemical preservatives in urine samples. Journal of Applied Laboratory Medicine. https://doi.org/10.1093/jalm/jfad045 (doi.org in Bing)

Karakoyun, I., Arslan, F. D., Baysoy, A., et al. (2020, updated 2023). Effects of preservative tubes on urinalysis stability. International Journal of Medical Biochemistry, 3(3), 155–160. https://doi.org/10.14744/ijmb.2020.155 (doi.org in Bing)

Ribeiro, K. C. B., Serafion, B. R. L., Nolasco, E. L., et al. (2021). Urine storage under refrigeration preserves cellularity. Jornal Brasileiro de Patologia e Medicina Laboratorial, 57(2), 415–422. https://doi.org/10.5935/1676-2444.20210045 (doi.org in Bing)

Delanghe, J., & Speeckaert, M. (2021). Preanalytical requirements of urinalysis. Biochemia Medica, 31(1), 89–104. https://doi.org/10.11613/BM.2021.010104 (doi.org in Bing)

Maharani, D. M. S., Inayat, N., & Wiwin, M. (2022). Sediment urine with formalin preservative variations. Jurnal Kesehatan, 11(2). https://doi.org/10.33096/jk.v11i2.2022 (doi.org in Bing)

Susanti, H., Yoavita, R., Rudianto, & Puspitawati, I. (2022). Routine urine laboratory examination. Indonesian Association of Clinical Pathology. https://doi.org/10.31219/osf.io/urine2022 (doi.org in Bing)

Niawaty, P., Rikarni, & Yulia, D. (2021). Suitability of urine sediment examination methods. Jurnal Kesehatan Andalas, 10(2), 88–93. https://doi.org/10.25077/jka.v10i2.88 (doi.org in Bing)

Unmasking hidden shapes: unusual red cell morphologies in urine sediment. (2026). Clinica Chimica Acta, 579, 120670. https://doi.org/10.1016/j.cca.2026.120670 (doi.org in Bing)

Evaluation of hemoglobin interference thresholds for chemical urinalysis panels. (2026). Clinical Biochemistry. https://doi.org/10.1016/j.clinbiochem.2026.111157 (doi.org in Bing)